Journal: Cell reports

Article Title: D1 and D2 neurons in the nucleus accumbens enable positive and negative control over sugar intake in mice

doi: 10.1016/j.celrep.2023.112190

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit-anti-mCherry Abcam RRID: AB_2571870 TRITC-conjugated affinipure Goat anti-Rabbit IgG (H + L) Jackson ImmunoResearch Labs RRID: AB_2337932 Goat Anti-GFP antibody (FITC) Abcam RRID: AB_305635 Anti-c-Fos Rabbit polyclonal to c-Fos Abcam RRID: AB_2231989 Biotinylated Goat Anti-Rabbit IgG antibody Vector Laboratories RRID: AB_2313606 Chemicals, peptides, and recombinant proteins Sucralose Sigma PubChem Substance ID: 329769983 Rebaudioside A Sigma PubChem Substance ID: 329747271 Glucose Sigma PubChem Substance ID: 24895335 Methyl α-D-glucopyranoside Sigma PubChem Substance ID: 24897328 Denatonium Benzoate Sigma PubChem Substance ID: 57654112 Lithium chloride Sigma PubChem Substance ID: 24896459 Victoza (liraglutide) injection Novo Nordisk N/A DiI lipophiliccarbocyanine dye Sigma PubChem Substance ID: 57650110 PubChem Substance ID: Invitrogen Cat #D1817 Dextran, Tetramethylrhodamine, 10,000 MW, Lysine Fixable (Fluoro-Ruby) Critical commercial assays VECTASTAIN Elite ABC HRP Kit (Peroxidase, Standard) Vector Laboratories Cat# PK-6100 C&B Metabond Parkell SKU: S380 Experimental models: Organisms/strains Mouse: B6.FVB(Cg)-Tg(Drd1-cre)EY262Gsat/Mmucd University of California, Davis MMRRC RRID:MMRRC_030989-UCD Mouse: B6.FVB(Cg)-Tg(Drd2-cre)ER44Gsat/Mmucd University of California, Davis MMRRC RRID:MMRRC_032108-UCD Mouse: B6(Cg)-Tg(Phox2b-cre)3Jke/J The Jackson Laboratory RRID:IMSR_JAX:016223 Mouse: B6; 129S6-Gt(ROSA)26Sortm9 (CAG-tdTomato)Hze/J The Jackson Laboratory RRID:IMSR_JAX:007905 Recombinant DNA and virus strains H129 CAG-LSL-TK-tdTomato NIH Center for Neuroanatomy with Neurotropic Virus ΔTK-TT PRV CMV-EGFP NIH Center for Neuroanatomy with Neurotropic Virus Ba-152 PRV CAG-DIO-TK-GFP NIH Center for Neuroanatomy with Neurotropic Virus Ba-2017 AAV-flex-taCasp3-TEVp Addgene Cat # 45580-AAV5; RRID:Addgene_45580 AAV-EF1a-DIO-hChR2(H134R)-EYFP Addgene Cat # 20298-AAV5; RRID:Addgene_20298 Software and algorithms MATLAB R2017b MathWorks https://www.mathworks.com/products/matlab/ GraphPad Prism 8 GraphPad https://www.graphpad.com/scientific-software/prism/ Adobe Illustrator CC 2017 Adobe https://shop.adobe.com/ Open Ephys GUI Open Ephys https://open-ephys.org/software Neuroscope, NDManager and Klusters Lynn Hazan & Michaël Zugaro http://neurosuite.sourceforge.net/ KlustaKwik Ken Harris http://klustakwik.sourceforge.net/ BukaLab Asapti Labs https://asapti.com/ BioRender BioRender https://biorender.com Other Implantable Optical Fibers Doric Lenses, Canada MFC_200/240–0.22_6mm_ZF1.25(G)_FLT 32 CH Microelectrode (Electrophysiological recordings) NeuroNexus A2x16–10mm-50–500-177-A32 Micro-Renathane (tubing) Braintree SKU: MRE033 Open in a separate window KEY RESOURCES TABLE.

Techniques: Plasmid Preparation, Recombinant, Injection, Software

Journal: Frontiers in Neuroinformatics

Article Title: DynaSim: A MATLAB Toolbox for Neural Modeling and Simulation

doi: 10.3389/fninf.2018.00010

Figure Lengend Snippet: Simulating a simple system of ordinary differential equations in DynaSim. (A) MATLAB code using the DynaSim toolbox. Simulation is achieved by passing a model specification to the DynaSim dsSimulate function. Simulated data are returned in a DynaSim data structure. (B) (x,z) phase plane of Lorenz system.

Article Snippet: Several features are not currently supported by GNU Octave including the DynaSim GUI, MATLAB Coder for MEX compilation, and parallel simulations using parfor .

Techniques:

Journal: Frontiers in Neuroinformatics

Article Title: DynaSim: A MATLAB Toolbox for Neural Modeling and Simulation

doi: 10.3389/fninf.2018.00010

Figure Lengend Snippet: Simulating an ODE system with conditional reset and stochastic drive. (A) MATLAB code using the DynaSim toolbox. The model is specified using a cell array of strings, eqns , listing equations defining parameters, an input function I(t) , ODEs with ICs, and a conditional reset. The stochastic input uses the built-in MATLAB function rand . (B) Plot of the time-varying input and simulated output.

Article Snippet: Several features are not currently supported by GNU Octave including the DynaSim GUI, MATLAB Coder for MEX compilation, and parallel simulations using parfor .

Techniques:

Journal: Frontiers in Neuroinformatics

Article Title: DynaSim: A MATLAB Toolbox for Neural Modeling and Simulation

doi: 10.3389/fninf.2018.00010

Figure Lengend Snippet: Simulating a biophysically-detailed neuron model using mechanisms. (A) DynaSim model leveraging existing model objects for iNaF, iKDR, and iM currents to simplify the specification of a detailed neuron model. (B) IB response to tonic current.

Article Snippet: Several features are not currently supported by GNU Octave including the DynaSim GUI, MATLAB Coder for MEX compilation, and parallel simulations using parfor .

Techniques:

Journal: Frontiers in Neuroinformatics

Article Title: DynaSim: A MATLAB Toolbox for Neural Modeling and Simulation

doi: 10.3389/fninf.2018.00010

Figure Lengend Snippet: Simulating weak PING rhythms using a model specification structure. (A) The conceptual object-based architecture of a biophysically-detailed network of excitatory (blue) and inhibitory (red) cells. (B) Mapping the object-based architecture onto a DynaSim specification structure that contains all the high-level information necessary to construct the complete system of equations for the full model using objects from a library of pre-existing ionic mechanisms.

Article Snippet: Several features are not currently supported by GNU Octave including the DynaSim GUI, MATLAB Coder for MEX compilation, and parallel simulations using parfor .

Techniques: Construct

Journal: Frontiers in Neuroinformatics

Article Title: DynaSim: A MATLAB Toolbox for Neural Modeling and Simulation

doi: 10.3389/fninf.2018.00010

Figure Lengend Snippet: Searching parameter space using the DynaSim toolbox. (A) MATLAB code using the DynaSim dsSimulate function with the vary option to specify a set of 9 simulations varying two parameters ( Iapp in population E and tauD of the connection from I to E). (B) Raster plots produced by dsPlot with the plot_type option given an array of DynaSim data structures containing results for all 9 simulations. (C) Plots produced by dsPlotFR showing how mean firing rates for E and I populations change as a function of the two varied parameters.

Article Snippet: Several features are not currently supported by GNU Octave including the DynaSim GUI, MATLAB Coder for MEX compilation, and parallel simulations using parfor .

Techniques: Produced

Journal: Frontiers in Neuroinformatics

Article Title: DynaSim: A MATLAB Toolbox for Neural Modeling and Simulation

doi: 10.3389/fninf.2018.00010

Figure Lengend Snippet: DynaSim Graphical User Interface showing the weak PING model.

Article Snippet: Several features are not currently supported by GNU Octave including the DynaSim GUI, MATLAB Coder for MEX compilation, and parallel simulations using parfor .

Techniques:

Journal: Frontiers in Neuroinformatics

Article Title: DynaSim: A MATLAB Toolbox for Neural Modeling and Simulation

doi: 10.3389/fninf.2018.00010

Figure Lengend Snippet: Object-based architecture, standardized specification, and DynaSim models. (A) Object-based architecture and standardized specification. Discrete model objects (populations and mechanisms) are shown in bold; any object can be stored independently in the library and reused as components of larger models. There is no limit on the number of objects in a DynaSim model. Fields of the standardized specification structure are underlined. Each population can have a list of intrinsic mechanisms; each directed pair of source and target populations can have a list of connection mechanisms. Optional objects are enclosed in parentheses. A string-based specification will be internally associated with a default population “pop1” in the standardized specification structure. (B) The standardized specification structure and model objects are parsed to generate a single set of equations describing the full model given the separate sets of equations for each object.

Article Snippet: Several features are not currently supported by GNU Octave including the DynaSim GUI, MATLAB Coder for MEX compilation, and parallel simulations using parfor .

Techniques:

Journal: Frontiers in Neuroinformatics

Article Title: DynaSim: A MATLAB Toolbox for Neural Modeling and Simulation

doi: 10.3389/fninf.2018.00010

Figure Lengend Snippet: Linking equations across population and mechanism objects. Mechanism linker statements with addition assignment (e.g., @current+=IK) direct DynaSim to substitute functions INa and IK into population-level dynamics “dv/dt,” where the linker appears (i.e., @current). In this example, intrinsic mechanisms are defined in script and added to specification structure in a mechanisms field.

Article Snippet: Several features are not currently supported by GNU Octave including the DynaSim GUI, MATLAB Coder for MEX compilation, and parallel simulations using parfor .

Techniques:

Journal: Frontiers in Neuroinformatics

Article Title: DynaSim: A MATLAB Toolbox for Neural Modeling and Simulation

doi: 10.3389/fninf.2018.00010

Figure Lengend Snippet: Single simulation workflow. From the user perspective, the functional interface to DynaSim involves specifying a model using strings or a DynaSim specification structure, passing it to dsSimulate , and obtaining a DynaSim data structure with the results of simulation. Internally, dsSimulate standardizes the supplied specification using the dsCheckSpecification function. The standardized specification structure is converted into a DynaSim model structure (Figure ) using the dsGenerateModel function, which adds object-specific namespace identifiers and links variables and functions across model objects (Figure ). A solve_file for numerical integration is automatically generated from the model structure by dsGetSolveFile according to simulator options. Simulated data is then obtained by evaluating the solve_file . DynaSim structures are shown in bold. Functions are followed by “().” Simulator options are enclosed in parentheses.

Article Snippet: Several features are not currently supported by GNU Octave including the DynaSim GUI, MATLAB Coder for MEX compilation, and parallel simulations using parfor .

Techniques: Functional Assay, Generated

Journal: Frontiers in Neuroinformatics

Article Title: DynaSim: A MATLAB Toolbox for Neural Modeling and Simulation

doi: 10.3389/fninf.2018.00010

Figure Lengend Snippet: Benchmarks. Time to simulate vs. network size for all benchmarks run; network sizes were 1, 2, 4, 8, 16, 32, 64, 128, 250, 500, 1,000, 2,000, 4,000, 8,000, 16,000, or 32,000 cells. Red lines indicate uncompiled Brian 2 simulation time for given network type and size, green lines indicate time for equivalent C++ compiled Brian 2 simulation, blue lines indicate time for equivalent DynaSim simulation without using MEX compilation, and black lines indicate time for equivalent DynaSim simulation using MEX compilation. (A) Benchmarks for simple “current-based” (CUBA) simulations consisting of cells containing just leakage currents and no synapses. (B) Benchmarks for Hodgkin-Huxley conductance-based (COBAHH) simulations of cells containing Na, K, and leakage currents and no synapses. (C) Benchmarks for COBAHH simulations, but with AMPA and GABA-A synaptic connections at a low density of 2% connection probability. (D) Benchmarks for COBAHH simulations, but with AMPA and GABA-A synaptic connections at a high density of 90% connection probability. Note that we could not simulate the highest-sized network (32,000 cells) using compilation under DynaSim, as the resulting data structures were found to be too large to be computed by MATLAB's compiling framework. DynaSim simulations using compilation worked successfully using network sizes of 16,000 cells, and those without compilation could successfully simulate 32,000 cells.

Article Snippet: Several features are not currently supported by GNU Octave including the DynaSim GUI, MATLAB Coder for MEX compilation, and parallel simulations using parfor .

Techniques: